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Cell Signaling Technology Inc c kinase 1
$a.) Heatmap of differentially expressed ribosomal proteins (adjusted P < 0.05) identified in the mitochondrial, nuclear, and cytosolic fractions. Colours indicate log□fold-change at each acute timepoint (MID, POST, 3H relative to PRE). b.) Scaled profile plots showing mean z-score changes of Receptor for Activated C <t>Kinase</t> <t>1</t> <t>(RACK1)</t> detected in the mitochondrial and nuclear fractions across PRE, MID, POST, and 3H. Subpanels illustrate trajectories for females (F), males (M) and combined sexes (F), obtained from the Subcell-EX Shiny app for visualising compartment- and sex-stratified comparisons. c.) Representative immunoblot images of RACK1 (5 µg total protein loaded per lane, including molecular weight marker and pooled control sample in the first lanes) across subcellular fractions (mitochondria, nucleus, cytosol) at PRE, MID, POST, and 3H. Lane colouring reflects fraction identity: mitochondria (peach), nucleus (blue), cytosol (green). A Stain-Free total protein image is shown below as a loading control. d) RACK1 subcellular protein abundance in response to exercise, determined using band densitometry and expressed in arbitrary units (a.u.). Quantified data are presented as mean ± SD for a representative subset of n = 10 participants (5 males, 5 females). Comparisons are shown for PRE, MID, POST, and 3H within each fraction (mitochondrial, nuclear, cytosolic). (*P ≤ 0.05; **P ≤ 0.001) relative to PRE, determined by pairwise t-tests with Bonferroni correction.$
C Kinase 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+kinase+1/RACK1+Rabbit+mAb/bio_rxiv__64898__2026__02__08__703653-248-3-7
Average 94 stars, based on 52 article reviews

c kinase 1 - by Bioz Stars, 2026-10

94/100 stars

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1) Product Images from "Subcellular proteomic profiling of human skeletal muscle reveals exercise-induced coordinated and compartment-specific protein remodeling"

Article Title: Subcellular proteomic profiling of human skeletal muscle reveals exercise-induced coordinated and compartment-specific protein remodeling

Journal: bioRxiv

doi: 10.64898/2026.02.08.703653

$a.) Heatmap of differentially expressed ribosomal proteins (adjusted P < 0.05) identified in the mitochondrial, nuclear, and cytosolic fractions. Colours indicate log□fold-change at each acute timepoint (MID, POST, 3H relative to PRE). b.) Scaled profile plots showing mean z-score changes of Receptor for Activated C Kinase 1 (RACK1) detected in the mitochondrial and nuclear fractions across PRE, MID, POST, and 3H. Subpanels illustrate trajectories for females (F), males (M) and combined sexes (F), obtained from the Subcell-EX Shiny app for visualising compartment- and sex-stratified comparisons. c.) Representative immunoblot images of RACK1 (5 µg total protein loaded per lane, including molecular weight marker and pooled control sample in the first lanes) across subcellular fractions (mitochondria, nucleus, cytosol) at PRE, MID, POST, and 3H. Lane colouring reflects fraction identity: mitochondria (peach), nucleus (blue), cytosol (green). A Stain-Free total protein image is shown below as a loading control. d) RACK1 subcellular protein abundance in response to exercise, determined using band densitometry and expressed in arbitrary units (a.u.). Quantified data are presented as mean ± SD for a representative subset of n = 10 participants (5 males, 5 females). Comparisons are shown for PRE, MID, POST, and 3H within each fraction (mitochondrial, nuclear, cytosolic). (*P ≤ 0.05; **P ≤ 0.001) relative to PRE, determined by pairwise t-tests with Bonferroni correction.$
Figure Legend Snippet: a.) Heatmap of differentially expressed ribosomal proteins (adjusted P < 0.05) identified in the mitochondrial, nuclear, and cytosolic fractions. Colours indicate log□fold-change at each acute timepoint (MID, POST, 3H relative to PRE). b.) Scaled profile plots showing mean z-score changes of Receptor for Activated C Kinase 1 (RACK1) detected in the mitochondrial and nuclear fractions across PRE, MID, POST, and 3H. Subpanels illustrate trajectories for females (F), males (M) and combined sexes (F), obtained from the Subcell-EX Shiny app for visualising compartment- and sex-stratified comparisons. c.) Representative immunoblot images of RACK1 (5 µg total protein loaded per lane, including molecular weight marker and pooled control sample in the first lanes) across subcellular fractions (mitochondria, nucleus, cytosol) at PRE, MID, POST, and 3H. Lane colouring reflects fraction identity: mitochondria (peach), nucleus (blue), cytosol (green). A Stain-Free total protein image is shown below as a loading control. d) RACK1 subcellular protein abundance in response to exercise, determined using band densitometry and expressed in arbitrary units (a.u.). Quantified data are presented as mean ± SD for a representative subset of n = 10 participants (5 males, 5 females). Comparisons are shown for PRE, MID, POST, and 3H within each fraction (mitochondrial, nuclear, cytosolic). (*P ≤ 0.05; **P ≤ 0.001) relative to PRE, determined by pairwise t-tests with Bonferroni correction.

Techniques Used: Western Blot, Molecular Weight, Marker, Control, Staining, Quantitative Proteomics

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Article Title: Subcellular proteomic profiling of human skeletal muscle reveals exercise-induced coordinated and compartment-specific protein remodeling
Article Snippet: Membranes were washed in TBS-T and incubated overnight with rolling at 4°C with primary antibodies of each subcellular compartment protein marker diluted in TBS-T. Antibodies used to verify compartment enrichment/purity included anti-histone (H3; Abcam #Ab1791; nuclear marker, 1:3000 dilution in 5% BSA), anti-mitochondrial heat shock protein 70 (mtHSP70; Invitrogen #MA3-028; mitochondrial marker, 1:1000), and anti-lactate dehydrogenase (LDHA; Cell Signaling Technology #2012; cytosolic marker, 1:1000). .. Receptor for Activated C Kinase 1 (RACK1, Cell Signalling Technology, #5432 CST) was also interrogated via immunoblotting to validate MS results. .. Following at least 3 x 10 min washes in TBS-T with vigorous rocking, membranes were incubated with HRP-conjugated secondary antibodies (goat anti-mouse IgM, Thermo Fisher #31172; goat anti-rabbit IgG, CST #707), diluted 1:5000 in 5% BSA.

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Journal: bioRxiv

Article Title: Subcellular proteomic profiling of human skeletal muscle reveals exercise-induced coordinated and compartment-specific protein remodeling

doi: 10.64898/2026.02.08.703653

Figure Lengend Snippet: a.) Heatmap of differentially expressed ribosomal proteins (adjusted P < 0.05) identified in the mitochondrial, nuclear, and cytosolic fractions. Colours indicate log□fold-change at each acute timepoint (MID, POST, 3H relative to PRE). b.) Scaled profile plots showing mean z-score changes of Receptor for Activated C Kinase 1 (RACK1) detected in the mitochondrial and nuclear fractions across PRE, MID, POST, and 3H. Subpanels illustrate trajectories for females (F), males (M) and combined sexes (F), obtained from the Subcell-EX Shiny app for visualising compartment- and sex-stratified comparisons. c.) Representative immunoblot images of RACK1 (5 µg total protein loaded per lane, including molecular weight marker and pooled control sample in the first lanes) across subcellular fractions (mitochondria, nucleus, cytosol) at PRE, MID, POST, and 3H. Lane colouring reflects fraction identity: mitochondria (peach), nucleus (blue), cytosol (green). A Stain-Free total protein image is shown below as a loading control. d) RACK1 subcellular protein abundance in response to exercise, determined using band densitometry and expressed in arbitrary units (a.u.). Quantified data are presented as mean ± SD for a representative subset of n = 10 participants (5 males, 5 females). Comparisons are shown for PRE, MID, POST, and 3H within each fraction (mitochondrial, nuclear, cytosolic). (*P ≤ 0.05; **P ≤ 0.001) relative to PRE, determined by pairwise t-tests with Bonferroni correction.

Article Snippet: Receptor for Activated C Kinase 1 (RACK1, Cell Signalling Technology, #5432 CST) was also interrogated via immunoblotting to validate MS results.

Techniques: Western Blot, Molecular Weight, Marker, Control, Staining, Quantitative Proteomics

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